What is Transcriptomics?
Transcriptomics is the study of the transcriptome or complete set of RNA. This study compiles expression levels of mRNAs in a specific cell population. When all of these expression levels are complied, you are able to understand where and the level of expression for your gene of interest [1]. Microarrays and RNA-seq can be used to study transcriptomics. RNA-seq tends to be preferred since it is more precise. These methods output sequences that are aligned by a reference genome [2]. One way to look at the transcriptome expression is to use The Human Protein Atlas website [3].
Discussion
The transcriptomic data reveals that the FH gene is expressed throughout the body in both females and males. In the body there is never no detection of FH in an organ or tissue. For the purposes of understand Fumarate Hydratases role in the kidney cell, we know regularly this activity is highly expressed. When the FH gene is knocked out, the cell now expressed low to no levels of Fumarate Hydratase which promotes carcinogenic activity in kidney cells [3].
Resources:
[1] Skerrett-Byrne Anthony, D., Jiang Chen, C., Nixon, B., & Hondermarck, H. (2022, November 25). Transcriptomics. Encyclopedia of Cell Biology (Second Edition). https://www.sciencedirect.com/science/article/abs/pii/B9780128216187001577
[2] Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009;10(1):57-63. doi:10.1038/nrg2484
[3]The Human Protein Atlas. (n.d.). FH. Tissue expression of FH - summary - The human protein atlas. https://www.proteinatlas.org/ENSG00000091483-FH/tissue
[2] Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009;10(1):57-63. doi:10.1038/nrg2484
[3]The Human Protein Atlas. (n.d.). FH. Tissue expression of FH - summary - The human protein atlas. https://www.proteinatlas.org/ENSG00000091483-FH/tissue
This web page was produced as an assignment for Genetics 564, a capstone course at UW-Madison.