What are Protein Domains?
Protein domains are the different units that make a protein. Each protein domain can function, fold, and evolve independently [1]. This means that each domain within a protein is unique and can be studied to understand protein interactions. Protein domains and protein interactions are a part of most biological functions within the cell [1]. You can use SMART and InterPro programs to identify protein domains of interest.
Discussion
The Domain analysis reveals the both N-terminal and C-terminal domains in FH are highly conserved across homologs. Understanding the role of the N-terminal and C-terminal will allow for precise knockouts WT zebrafish. If one or both knockouts of these domains occur and it results in mutant FH phenotype, this will give insight on FH function in the kidney cells.
References:
[1]Wang, Y., Zhang, H., Zhong, H., & Xue, Z. (2021). Protein domain identification methods and online resources. Computational and structural biotechnology journal, 19, 1145–1153. https://doi.org/10.1016/j.csbj.2021.01.041
[2] SMART. (n.d.). FH protein domains. http://smart.embl-heidelberg.de/smart/show_motifs.pl
[2] SMART. (n.d.). FH protein domains. http://smart.embl-heidelberg.de/smart/show_motifs.pl
This web page was produced as an assignment for Genetics 564, a capstone course at UW-Madison.